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Message | User | Date(yyyy-mm-dd) |
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HUVEC morphology | rharfo | 2001-12-04 | Click here to register. |
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I and others have had a weird experience with HUVECs, and we are running out of hypothesis.
After plating, there seems to be more ?granules in the supernatant then ordinarly, and many of the cells adopt a rounded morphology (enlarged spherical cytoplasm). The most puzzling item is that when cells were plated on LOW surface areas (9.6cm2 and below, at the recommended density, they changed morphology as sooon as they were fed the next day, forming numerous vacuoles (flower shaped!).The latter is prevented when cells are plated at a higher density than recommended.
What's more, all our other cells are in an impecable state (hence, ?not contamination).
We would really appreciate any kind of help!
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| jpartee | 2001-12-05 | Click here to register. |
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 | That could be any number of things from contamination to bacterial contamination. You can send your cells to any of the cell culture collections, like ATCC and they can do bacterial testing as well as some genetic and morphology comparisons. |
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