There's a sale on Spectrum's Products, 10% off Laboratory Products!

CellCultureChat.com:   The Answers for All of Your Cell Culture Questions.


Close MessageUserDate(yyyy-mm-dd)
Previous Subject
Next Subject 		Is DMSO toxic to cellskavitha2009-01-19Click here to register.
i'm working with rat skeletal myoblast cell line(L6). i'm using the fluorescence probes. it should be dissolved DMSO (100% DMSO). I'm using 2.3 microliter dmso in 1ml DMEM medium. my cells are not looking happy after the incubation with the DMSO dissolved dye. they all become rounded and going to dye. can any one tell me is that because of DMSO or I'm using ringer buffer while imaging the cells. is the ringer buffer composition matters? i would be grateful if i get the reply.
 
Re: Is DMSO toxic to cells
lubrol2009-02-17Click here to register.
Kavitha;

Yes, DMSO can be toxic to the cells; to check this, place 2.23 microliters of fresh DMSO without the probes on some myoblasts and look for the same reaction. If you don't see it, it may be the probes or old DMSO. Also, the composition of the Ringers can also play a role. The normal composition is:
8.6 g of NaCl, 0.3 g of KCl, and 0.33 g of CaCl2 in each 1000 ml of distilled water
Sometimes, lactated Ringer' is used instead for myoblasts/myoblasts, It has a composition of:
Sodium Chloride 6 g, Sodium Lactate 3.1 g, Potassium Chloride 300 mg, Calcium Chloride • 2H2O 20 mg brought to 1L in distilled water.

Hope this helops,

Lubrol

Thanks for visiting.
  • To ask or answer a question, you need to register here.
  • You can browse by clicking on a message subject to see the complete message.
  • If you have previously registered, you can log in below.

Message (click on a subject to view the messages)
Click for older messages
OlderOlder LastOldest
Click for newer messages
NewestFirst LastNewer
Search for messages that contain  

You must register before asking a question or providing an answer.
You can click here to register. If you have already registered you can log in below.
(If you check the Use Cookies box then your computer will remember your log in name.)
User Name:
Password:


Click here if you previously registered and remember your user name but not your password.
Email us at cccc@lplc.com if you are having other problems.


Back to Top