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Next Subject 		Freezing of cellsRen2008-10-31Click here to register.
Hi,

This is my first time posting and i am also quite new to cell culture. I just realized that the medium of the cells that i froze last nite turned into yellow. Does that mean that all my cells in the vial are dead? It happened to other previous cells that i've frozen too, but the medium turned into bright pink instead. Can someone tell me what's with the colour changes and do you think my cells are still alive? And how can i avoid this in the future?

I should have try thawing them and see whether they are still alive but i just want to get myself ready to face the disaster cause these cells are my precious :(

I hope someone can help me.

Thanks in advance!
 
Re: Freezing of cells
JAR2008-10-31Click here to register.
That is normal. If you want more information on cell Freezing, the Corning Life Sciences web site (www.corning.com/lifesciences) has both a detailed protocol and a technical brochure outline the hows and whys of cell freezing.
 
Re: Freezing of cells
Ren2008-11-01Click here to register.
Thanks heaps!

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