CellCultureChat - THP-1 culture problem

CellCultureChat.com: The Answers for All of Your Cell Culture Questions.

Message

User

Date(yyyy-mm-dd)

THP-1 culture problem

2008-05-22

Click here to register.

Hello,

I have been using THP-1 cells since November but now I defreezed them and I have a different behavior as before: one part of cells grow in suspension forming aggregates and the other part are differentiated adherent cells. Before I only had suspension and single cells. Could anyone tell me if these cells grow in a normal way? If I have adherent cells, could it be that I have a bacterial contamination since I also observed some small black points in the medium ?
Thank you for your answers

	Re: THP-1 culture problem

gbm

2008-06-02

Click here to register.

Hi,
Normally THP-1 cells show more aggregates in first 2 sub cultures, afterwards you can see clear single cells, this is not a problem.coming to differentiated adherent cells; i never faced this problem, only if you add PMA they turn to macrophage phenotype.just try with another frozen tube and use freshly prepared medium. all the best.

	Re: THP-1 culture problem

2008-06-03

Click here to register.

Hi,
thank you very much for your answer. I will try with another frozen tube.
Best Regards

Thanks for visiting.

To ask or answer a question, you need to register here.
You can browse by clicking on a message subject to see the complete message.
If you have previously registered, you can log in below.

Message (click on a subject to view the messages)

microbiology by mohamed on 2008-06-03
Collagen staining by n@@n! on 2008-05-31

Re: Collagen staining by seanpeel on 2008-06-03

hybridoma by kuhi on 2008-05-22

Re: hybridoma by n@@n! on 2008-05-31

THP-1 culture problem by BF on 2008-05-22

Re: THP-1 culture problem by gbm on 2008-06-02

Re: THP-1 culture problem by BF on 2008-06-03

stability problem with EGCG by ekz on 2008-05-14

Re: stability problem with EGCG by n@@n! on 2008-05-31

CANCER CELLS by S.SHIRBAZOU on 2008-05-13
CANCER CELL CULTURE by S.SHIRBAZOU on 2008-05-13
endotoxin by shanaki on 2008-05-12

Re: endotoxin by weedgiedg on 2008-06-18

activity of trypsine used for cell culter\cel dissociation by amol on 2008-05-11
phyto culture by frans on 2008-05-10
Using LB Broth to check for contamination by emma54 on 2008-04-30

Re: Using LB Broth to check for contamination by sferg on 2008-05-05
Re: Using LB Broth to check for contamination by n@@n! on 2008-05-06

How to use Robiot HEPES in cell culture? by li2008 on 2008-04-26
dendritic cell culture by sandra on 2008-04-24
cell culture versus breast cancer by robermann on 2008-04-22

Re: cell culture versus breast cancer by sferg on 2008-05-05

Process for tanning red blood cells by starbuck099 on 2008-04-17

Older	Oldest
Click for older messages

Newest	Newer
Click for newer messages

You must register before asking a question or providing an answer. You can click here to register. If you have already registered you can log in below. (If you check the Use Cookies box then your computer will remember your log in name.)
User Name:
Password:

Click here if you previously registered and remember your user name but not your password. Email us at cccc@lplc.com if you are having other problems.

All of the comments made are the property and responsibility of the person making them. They are not the responsibility of Spectrum.
Please direct all questions and comments to us at moderator@cellculturechat.com.
General information on Spectrum's filters and filtration products is available at http://www.spectrapor.com/0/0/7.html.
Have a filtration question? Try www.filterchat.com.
Need a Fraction Collector? see www.lplc.com.