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Message | User | Date(yyyy-mm-dd) |
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trpsinisation | mystery | 2008-04-10 | Click here to register. |
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| what is the difference between trypsinisation at 4 c and 37 c,please? | | |
| lubrol | 2008-04-10 | Click here to register. |
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 | There is no essential difference between 4° C and 37° C . In both case the procedure enzymatically cleaves the extracellular portion of various integrins and other attachment proteins and components of the extracellular matrix. This allows adherent cells to be released from the culture surface. The real difference is the rapidity of the action and as a result the extent and nature of the proteins cleaved. For some cells the lower temperature will result in higher viability and faster reattachment; for others there will be insufficient action to release the majority of the cells from the surface. This will be partially cell line dependent and partially dependent on the culture conditions. As a general rule, lower temperatures are favored for cells that have difficulty attaching and or where cell surface receptors are being studies. In some cases, Versene (EDTA solution) is preferable to trypsinization or even physical scraping.
A lot depends on what the cells are to be used for, growth conditions, and the cells themselves - whatever the provider of the cell line recommends is the place to start. |
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