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Message | User | Date(yyyy-mm-dd) |
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cell cryogenic storage | mjsousa3 | 2008-02-20 | Click here to register. |
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what is the best way to store cell banks, in the liquid Nitrogen or in the Vapor phase??
Also does anyone have experience with validating the vapor phase storage units, and if so what is concidered "critical temperature" for cell storage? | | |
| lubrol | 2008-02-21 | Click here to register. |
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 | Vapor phase is considered the preferred storage location as it prevents cross-contamination (low probability but documented to happen) and vial explosion when removed from liquid phase (this is more of a problem with plastic vials, but glass ones can also leak and are much more dangerous if they explode)
The "critical" temperature is generally accepted as -135 C: at or below this temperature all of the water in the cell is frozen.
You can find more information on this on the Nunc (http://tinyurl.com/3bol8o - look for the ""Safety first - Nunc Cryopreservation Manual") and the Corning Life Sciences websites (at http://tinyurl.com/2rnvrd and http://tinyurl.com/2vn8qy ).
I'm not clear on what you mean by "validating vapor phase storage units". Most of this would be handled by the supplier in terms of a validated product; after that, the use of the unit becomes more of an issue of developing a written protocol, training people to the protocol and tracking compliance to the protocol. Sorry if that's pretty general but validation is so situation specific.
Lubrol
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