The CF-1 Fraction Collector, a laboratory workhorse.

CellCultureChat.com:   The Answers for All of Your Cell Culture Questions.


Close MessageUserDate(yyyy-mm-dd)
Previous Subject
Next Subject 		Neuro2a and black particlesNits2008-02-05Click here to register.
From a long time, I’m facing a problem in growing Neuro2a.I’m getting black particles floating in the media over the cell monolayer. It doesn’t appear like a cell debris to me. I think it is a contamination because it increases with time but don’t know what type of contamination it is? I’m adding amphotericin B and Penicillin/ Streptomycin in the media.
Please help.
 
Re: Neuro2a and black particles
JAR2008-02-16Click here to register.
It is most likely cellular debris not contamination. The best way to determine whether it is biological or not is to do a fluorescent DNA test. This is usually used for mycoplasma testing but will work here as well. For a protocol go to www.corning.com/lifesciences click technical information, click cell culture and look under protocols.
John

Thanks for visiting.
  • To ask or answer a question, you need to register here.
  • You can browse by clicking on a message subject to see the complete message.
  • If you have previously registered, you can log in below.

Message (click on a subject to view the messages)
Click for older messages
OlderOlder LastOldest
Click for newer messages
NewestFirst LastNewer
Search for messages that contain  

You must register before asking a question or providing an answer.
You can click here to register. If you have already registered you can log in below.
(If you check the Use Cookies box then your computer will remember your log in name.)
User Name:
Password:


Click here if you previously registered and remember your user name but not your password.
Email us at cccc@lplc.com if you are having other problems.


Back to Top
All the Perl that's Practical to Extract and Report