CellCultureChat - cell density

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cell density

Lena

2008-01-21

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How one can increase the cell density of the CHO cells ?

	Re: cell density

seanpeel

2008-02-20

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We have successully used Integra Biosciences CELLine CL-350 flasks which are a perfusion based system to increase cell density in the cell compartment. We can achieve 25 million cells/ml (200+ million per flask), compated to 2 million/ml(20 million/flask) in a T-75.

Note that the cells in teh CL-350 are in a compartment containg 5-9 mls of medium, but that there is a medium reservoir containing a further 340mls of medium. Your recombinant protein is retained in the cell compartment making it easier to purify.
see
http://www.integra-biosciences.com/celline_1_e.html
for more information

	Re: cell density

Lena

2008-02-26

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Thank you very much Seanpeel ,but I think I will not be able to use these flasks as they must be meant for adherent cell lines & my cell lines are adopted to suspension.

	Re: cell density

seanpeel

2008-02-26

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Hi Lena:

The CL series flasks are for suspension culture, the AS-series flasks are for adhesion. You can definately use the CL-350 or CL-1000 flasks for suspension cultures (we have !)

Sean

	Re: cell density

Lena

2008-02-27

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Thanks a lot Sean ,I will look for more information on this.

Lena

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cell density by Lena on 2008-01-21

Re: cell density by seanpeel on 2008-02-20

Re: cell density by Lena on 2008-02-26

Re: cell density by seanpeel on 2008-02-26

Re: cell density by Lena on 2008-02-27

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