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Next Subject 		Why/how does cell culture change cells?Jay2001-08-20Click here to register.
Hi -
Can you tell me what causes cells to change
over time in culture? That is, they become
aneuploid, lose contact inhibition, etc., why?

Thanks,
Jay Armstrong
 
Re: Why/how does cell culture change cells?
jpartee2001-08-21Click here to register.
Cosmic Radiation can cause mutations which effect the cells.  Physical changes in DNA, like breaking strands can effect the cells (although this rarely happens). When DNA replicates, it does not replicate 100% correctly. Proof reading of the DNA by polymerase fixes most errors but some can slip through and effect the cells.

Viruses can also infect the cells and change the DNA.

All of these sources can cause changes in DNA which can change properties of the cells.

Cells in the body are monitored by neighbor cells and the immune system. When a cell becomes mutated, it either destroys itself, apoptosis, or is attacked and killed by the immune system.

A culture dish does not have an immune system so one rogue cell can multiply prosper and rule with an iron fist.

Remember, there is never one reason that a cell changes properties (phenotype or genotype) either in vivo or in vitro.

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