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Message | User | Date(yyyy-mm-dd) |
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cell density | Lena | 2008-01-21 | Click here to register. |
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How one can increase the cell density of the CHO cells ?
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| seanpeel | 2008-02-20 | Click here to register. |
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 | We have successully used Integra Biosciences CELLine CL-350 flasks which are a perfusion based system to increase cell density in the cell compartment. We can achieve 25 million cells/ml (200+ million per flask), compated to 2 million/ml(20 million/flask) in a T-75.
Note that the cells in teh CL-350 are in a compartment containg 5-9 mls of medium, but that there is a medium reservoir containing a further 340mls of medium. Your recombinant protein is retained in the cell compartment making it easier to purify.
see
http://www.integra-biosciences.com/celline_1_e.html
for more information |
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| Lena | 2008-02-26 | Click here to register. |
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| Thank you very much Seanpeel ,but I think I will not be able to use these flasks as they must be meant for adherent cell lines & my cell lines are adopted to suspension.
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| seanpeel | 2008-02-26 | Click here to register. |
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| Hi Lena:
The CL series flasks are for suspension culture, the AS-series flasks are for adhesion. You can definately use the CL-350 or CL-1000 flasks for suspension cultures (we have !)
Sean |
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| Lena | 2008-02-27 | Click here to register. |
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| Thanks a lot Sean ,I will look for more information on this.
Lena |
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