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User

Date(yyyy-mm-dd)

BHK21

abobakr

2007-12-02

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I have aproblem in subculturing BHK21.clumps are formed after adding trypsin EDTA.The cells are degenrated after 2 to 3 passages.

	Re: BHK21

anildsgs

2007-12-19

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hi,

try to use 0.1 to 0.5% conc trypsin, as the BHK21 are very sensitive..........lead to clumps and after washing the cells with pbs try to pass throgh 18 gaze needdle fro the cell separation. I think it might help you to get rid o fthe clumps.

Anil

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BHK21 by abobakr on 2007-12-02

Re: BHK21 by anildsgs on 2007-12-19

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