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Next Subject 		BHK21abobakr2007-12-02Click here to register.
I have aproblem in subculturing BHK21.clumps are formed after adding trypsin EDTA.The cells are degenrated after 2 to 3 passages.
 
Re: BHK21
anildsgs2007-12-19Click here to register.
hi,

try to use 0.1 to 0.5% conc trypsin, as the BHK21 are very sensitive..........lead to clumps and after washing the cells with pbs try to pass throgh 18 gaze needdle fro the cell separation. I think it might help you to get rid o fthe clumps.

Anil

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