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Dependence of Young Hybridomas on Hybridoma Cloning Factordianewerth2007-09-20Click here to register.
I have just developed some Mouse Hybridoma Cell Lines. Fusion partner is Ag8. I would like to decrease the Hybridoma Cloning Factor supplementation gradually after the first round of subcloning to prepare a production grade cell line. Does anyone have experience with stability issues during HCF weaning or know of any references offhand. Thanks, Diane

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