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Next Subject 		cell counting with methylene bluecarren2007-10-25Click here to register.
Hi,
Does any one know the protocol for counting cells with a haemocytometer and methylene blue? can it be done in the same way as trypan blue or do the cells have to be fixed? (im using suspension cells)

Thanks
 
Re: cell counting with methylene blue
davidj2007-10-25Click here to register.
Carren,

http://ajpcell.physiology.org/cgi/content/full/286/6/C1390

this might give you an idea, however i would not recommend it as an assay for viability in moast mammalian cells.
 
Re: cell counting with methylene blue
JAR2007-10-25Click here to register.
Methylene blue with citric acid was used to lyse cells and leave their nuclei intact and stained so they could be counted on a hemacytometer. This technique was developed before they had trypsin so cells were scraped rather than released by enzymes during harvesting. It is still a good approach to count cells that are clumped.

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