The CF-1 Fraction Collector, a laboratory workhorse.

CellCultureChat.com:   The Answers for All of Your Cell Culture Questions.


Close MessageUserDate(yyyy-mm-dd)
Previous Subject
Next Subject 		Media quality amongst different companiesgpapadi2007-09-20Click here to register.
I have recently heard from several users that Hyclone and Gibco media yielded more vital cultures for them than when the equivalent media was used from Mediatech. The formulations appeared to be identical to the Mediatech recipe, yet the attached cells that the users were growing appeared to thrive in the other two versions. Has anyone made similar observations?
 
Re: Media quality amongst different companies
trish2007-09-23Click here to register.
There can be differenced in the same media formulation prepared by different suppliers....however there can be differences from one lot to the next from the same supplier. The differences are often caused by differeneces in the components used in the media. I have experince auditing both Gibco and Hyclone (as well as other media suppliers) and know that they have extensive raw materials testing programs to make sure that their raw materials provide similar growth from one lot to the next. I have experience with many of the other media suppliers and know that they have the same types of programs....however I don't know if Mediatech has that type of program in place. They should be willing to share with you their raw materials testing program and the results of their growth assays on each and every lot that they produce.

There has been a persistent rumor for more that the past 5 years that Mediatech produces media that doesn't grow cells well yet I have never seen one iota of data to back that up. If I had concerns that the media was inferior I would set up my own comparison with several different cell lines making sure that the forumations were truely identical. To that extent I would make sure that the formulations being tested were glutamine free and were supplemented just before use. I would also try to obtain media that have the same expiration dates.

So I guess to answer your question....I have heard the story several times but I have never been able to get anyone to show me data demonstrating the problem...

Trish

Thanks for visiting.
  • To ask or answer a question, you need to register here.
  • You can browse by clicking on a message subject to see the complete message.
  • If you have previously registered, you can log in below.

Message (click on a subject to view the messages)
Click for older messages
OlderOlder LastOldest
Click for newer messages
NewestFirst LastNewer
Search for messages that contain  

You must register before asking a question or providing an answer.
You can click here to register. If you have already registered you can log in below.
(If you check the Use Cookies box then your computer will remember your log in name.)
User Name:
Password:


Click here if you previously registered and remember your user name but not your password.
Email us at cccc@lplc.com if you are having other problems.


Back to Top
All the Perl that's Practical to Extract and Report