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Message | User | Date(yyyy-mm-dd) |
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horse serum | dpsarathi_9 | 2007-09-19 | Click here to register. |
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| I thawed first time one of my horse serum bottle(sigma)(donor herd) for my current work, but it was observed that it is looking like turbid appearance (white in color) and some flocculation also observed. please help me what could be the problem ? | | |
| trish | 2007-09-23 | Click here to register. |
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 | What you are seeing is protein and lipids precipitating out of the serum because of the freezing and thawing. Horse serum is much worse about this that any other type of media that I have worked with.....the serum also tends to have a greenish tinge to it that I don't see with other serum types.
I have found that it varies from one lot to the next. If I quick thaw the serum I tend to have less precipitation. If I repeatedly freeze and thaw I get less precipitation and cell line differentiation that I am trying to get in the HS is less.
What I have resorted to doing is buying small bottles, thawing once and alloquoting, using the serum with the precipitate.
Trish |
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| dpsarathi_9 | 2007-09-23 | Click here to register. |
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| Thank you trish, We have only 500ml bottles. I was warried that it was contaminated. What would you suggest now, should I use this serum assuch or any method is there (other than filteration) to remove protein from serum. I am planning to use this for embryonic stem cell work.
thank you once again
sarathi |
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| trish | 2007-10-07 | Click here to register. |
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| If you don't want to filter it you can try centrifugation.....it should get the bigger stuff. I find that I can't see the presipitate (other than the globular looking patrs) once it is diluted in media and the media is warmed to 37.
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