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eGFP mouse marrow cells will FACS analysis be a problem?purush_792007-07-26Click here to register.
Hi All,

I am using transgenic eGFP mouse marrow cells for my work and I want to characterize them with specific markers.

I intend to do so using direct conjugated primary antibodies.

If my antibodies are FITC conjugated..I suspect there would be a problem to detect the antibody signal with the GFP fluoroscene!!

Is there an alternative for this with out switching to a different fluorochrome?

Thanks in advance.

Purushotham Reddy

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