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Message | User | Date(yyyy-mm-dd) |
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eGFP mouse marrow cells will FACS analysis be a problem? | purush_79 | 2007-07-26 | Click here to register. |
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Hi All,
I am using transgenic eGFP mouse marrow cells for my work and I want to characterize them with specific markers.
I intend to do so using direct conjugated primary antibodies.
If my antibodies are FITC conjugated..I suspect there would be a problem to detect the antibody signal with the GFP fluoroscene!!
Is there an alternative for this with out switching to a different fluorochrome?
Thanks in advance.
Purushotham Reddy |
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