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Next Subject 		HepG2 cultureRajie2007-07-10Click here to register.
helo, i am culturing HepG2 as well as WRL cells.the cells were contaminated by bacteria.therefore, i used thawed cells to continue my project.however, after a limited time(about 3-5days) my cells were contaminated again.plz help me wt some idea why does this happen. i use DMEM media with 10%FBS,1%Pent/Strep and 1% of L-glutamine.It is possible that the bacteria were resistant to antibiotics??????
 
Re: HepG2 culture
doriana.t2007-07-26Click here to register.
Yes...It's possible. You could try adding Gentamycine. Bye
 
Re: HepG2 culture
sabaee2007-08-27Click here to register.
No,but your cocentration of pen must be 100 microgram per mil.

Cleaning hood is very important, spray disinfect walls and bottom and then leave it without wipp it with paper.

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