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Next Subject 		Cartridge Reuse?moderator2001-03-19Click here to register.
Can I reuse or autoclave the cartridges?
 
Re: Cartridge Reuse?
moderator2001-03-19Click here to register.
No. Re-use of the cartridge with a different cell line is not recommended. Once the cells have been loaded into the cartridge they will attach to the fiber and lay down a basement membrane. If you autoclave the cartridge at this point in time you will clog up the fibers. If you contaminate the cartridge during the pre-culture phase (before you have loaded cells) you can autoclave the cartridge. The flow path itself is not autoclavable. Alternative methods of sterilization must be used such as ETO.

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