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Message | User | Date(yyyy-mm-dd) |
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growth-rate of WiDr (human colon-adeno-carcinoma cell line) | Seven | 2001-08-10 | Click here to register. |
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| When determining the doubling time of my culture, does it matter how much cells I start with? The doubling time is theoretically 15 hours, but I've had results ranging from 13 to 20 hours. Can any one help me? | | |
| jpartee | 2001-08-10 | Click here to register. |
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 | Doubling time is the average time it takes for the cells to double. The amount does not matter normally. However, as cells grow, they sometimes
excrete a growth factor that helps improve growth. This might increase doubling time but I think that it just helps keep the cells alive longer which gives the appearance of faster growth (since you are not counting as many dead cells). |
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