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Next Subject 		J774 macrophagesclairemcloug2001-08-08Click here to register.
Why is it that the murine cell line J774.2 can be overstimulated eg by a cytokine mixture to a point where a drug/inhibitor fails to work?
 
Re: J774 macrophages
jpartee2001-08-08Click here to register.
I do not have an answer to your question.  I do have some references for J774.1 that might help.
(Obtained from ATCC.)

RF10973: Ralph P et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976
PubMed: 76192838

RF11022: Ralph P and Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977
PubMed: 77250888

RF11023: Ralph P and Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977
PubMed: 77089303

RF33245: Ralph P et al. Reticulum cell sarcoma: an effector cell in antibody-dependent cell- mediated immunity. J. Immunol. 114: 898-905, 1975
PubMed: 75096209

RF33307: Ralph P and Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257: 393-394, 1975
PubMed: 76030195

RF42547: Knowlton KU et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996
PubMed: 97048061

RF42725: Schissel SL et al. Zn2+-stimulated sphingomyelinase is secreted by many cell types and is a product of the acid sphingomyelinase gene. J. Biol. Chem. 271: 18431-18436, 1996
PubMed: 96324911

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