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Message | User | Date(yyyy-mm-dd) |
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HUVEC Cell line enquiry | ruby | 2001-06-20 | Click here to register. |
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HUVEC Cell ine enquiry
Can I culture HUVEC cell line without using any specialized endothelial enrichment media or it can be grown using the more commonly available media like DMEM,F12 or RPMI etc.
How far will be the vialbility if it is grown as latter.
With thanks,
Ruby Dhar
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| jpartee | 2001-06-20 | Click here to register. |
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 | Unless you have somehow adapted the HUV-EC-C to be grown without the special enriched media, it is not recommended that you change the media "cold turkey". Cells do not like to have nutrients or media changed without sufficient time to adapt to the new media. Most HUV-EC-C cells that I have seen or worked with needed the special media, usually Heparin and ECGF growth factors added. If you change the media, the viability can be as low as zero, it depends on the cell line. Since these cells only double about 60 times, it is best if you just use the media that they require and not change the formulation.
If you are using the Cellmax system, add the heparin and the ECGF directly to the ECS. Add it at regular intervals, usually when you harvest the
cells.
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