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Message | User | Date(yyyy-mm-dd) |
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amniotic cell culture | claudiamures | 2010-01-10 | Click here to register. |
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Hello!
I have some problem with amniotic cell culture. Amniotic cells did not adhering to culture vessel (amniodish from Euroclone and flask.
The CO2 level seems do be ok, i had sterilisesd de CO2 incubator, and after 2, 3 5 days the cell are floating inthe medium and and none of them is adherent.
I used the same medium (Amniochrome from Lonza), the same culture vessels. Everything was ok till 6 weeks ago when thw problwm had appear.
Thanks a lot.
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| wtamashiro | 2010-01-13 | Click here to register. |
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 | Thanks for your question. The technical people at Spectrum would like to discuss the issue in more detail to see if there is a solution. Please call
310-885-4600 and ask for tech service or e-mail techservice@spectrumlabs.com |
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| claudiamures | 2010-01-13 | Click here to register. |
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| Hello, I found the problem of amniotic cell culture failure. It was CO2 pressure regulator, it was broken(unfortunatelly 2 engineers didn't notice that).
Thanks a lot
Claudia |
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| wtamashiro | 2010-01-14 | Click here to register. |
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| It is good to hear you fixed the problem. If you have any other cell culture questions please feel free to contact Spectrum. |
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