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Next Subject 		cell culturemystery2008-04-10Click here to register.
what is the difference between trypsinisation at 4 c and trypsinisation at 37 c?
 
Re: cell culture
sferg2008-05-05Click here to register.
the increased temperature (37'c vs 4'c) will increase the activity of the trypsin. some cell lines are more sensitive to this enzyme and therefore are trypsinized at 4'c, to prevent damage to cells

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